Factors influencing the plant regeneration frequency in indica rice callus cultures

Using immature embryos of indica rice cultivar Teqing as explants, we investigated factors influencing plant regeneration. The results showed that MS+2.4- D 2 mg/L was the optimal induction medium and that pre -differentiation culture, dehydration treatment, sufficiently air - exchangeable state of the vessels, and decreasing sucrose concentration of differentiation medium could significantly increase the regeneration frequency of rice callus.