Significance of Expression of Monocyte Chemoattractant Protein-1 in Rats Model of Doxorubicin-Induced Nephropathy

ObjectiveTo observe the expression of monocyte chemoattractant protein-1(MCP-1)in renal tissue and urine of Doxorubicin-induced nephropathy rats,and to discuss its possible mechanism in minimal change nephrotic syndrome(MCNS).MethodsEighty-three male Wistar rats were enrolled in this study.They were randomly divided into normal control group(n=40) and nephropathic group(n=43).The nephropathic group was established by a single intraperitoneal injection of Doxorubicin 5 mg/kg.The 24 h urine protein of all rats was measured on the 7th,14th,28th and 42th day.The MCP-1 level in renal tissue and urine were determined with ELISA.SPSS 11.0 software was used to obtain t-test of various groups and correlation analysis of 2 variables.Results1.The 24 h urine protein of nephropathy rats increased gradually on the 7th,14th and 28th day,and it was greatly higher than that of normal control group(Pa0.01).2.The MCP-1 level in renal tissue and urine of nephrotic group each time point was significantly increased than that of the normal control group(Pa0.01).3.The MCP-1 in urine and 24 h urine protein concentrations were positively correlated(r=0.651P0.01).The MCP-1 level in renal tissue and 24 h urine protein concentrations were positively correlated(r=0.583P0.01).In kidney and urinary concentrations of MCP-1 was positively correlated(r=0.810P0.01).ConclusionsMCP-1 involved in the incidence of proteinuria in rats with Doxorubicin-induced nephropathy.MCP-1 level may reflect the MCP-1 level of renal tissue,and as an index to judge MCNS condition.