Effects of sinomenine on doxorubicin nephropathic rats: An experimental study

Objective:To observe the interleukin-6(IL-6) expression in the renal tissue and urine of doxorubicin nephropathy rats and the effect of Sinomenine(SIN) on its expression.Methods: We divided 120 male Wistar rats randomly into normal group(32) and nephropathy group(88).A single intravenous injection of doxorubicin 5mg/kg was given to nephritic rats to prepare with nephropathy(MCNS) model.We used enzyme-linked immunosorbent assay(ELISA) to detect the IL-6 expression in the different periods of the renal tissue and urine.Results:①Nephritic rat urine protein was gradually increased,with a significant difference compared with the normal group(P0.01).②The IL-6 expression in the kidney tissue and urine of the nephropathy group was significantly increased compared with the normal group,while that of the treatment group was lower than that of the nephropathy group.③The IL-6 expression in the kidney tissue and urine of the nephropathy group was positively related to the 24 h urine protein(R=0.870,0.794 P0.01).4.The IL-6 expression level in the kidney tissue was positively correlated with that in the urine(R=0.739,P0.01).Conclusion:①Nephritic rats urine and renal tissue IL-6 levels are significantly increased,positively correlated with the 24h urine protein level,suggesting that IL-6 and doxorubicin nephropathy is closely related to the occurrence of proteinuria.②The IL-6 expression level in the kidney tissue was positively correlated with that in the urine.③SIN could reduce proteinuria by downregulating the IL-6expression in MCNS,which plays a role in kidney protection.