2010•Pharmaceutical Care and ResearchRequires access
Effect of Qinshixunjing patch on experimental asthma in guinea pigs
Chenhuan Ding, Yiqun Li, Jie Gong, Wentao Yang, Rui Bi
Open publisher page 0 citations
Abstract
Objective:To investigate the effect of Qinshixunjing patch on experimental asthma in guinea pigs.Methods:Thirty guinea pigs were randomly divided into normal control group(group A),asthma model group(group B) and Qinshixunjing patch group(group C),10 in each group.The asthmatic models were established by ovalbumin sensitizing and challenging method.The guinea pigs in group C were treated with external application of Qinshixunjing patch.The guinea pigs in group B were treated with gauze patch containing saline.The total white blood cell count,the percentage of eosinophils and neutrophils in peripheral blood were calculated after application of test patch.The lung tissues and bronchi were stained with hematoxylin-eosin(HE) to observe the pathological changes.Results:Compared with group A,the percentage of eosinophils in peripheral blood in group B increased significantly(P0.05).The percentage of eosinophils in group C was significantly lower than that in group B(P0.05).There was no significant difference in the percentage of neutrophils and total white blood cell count among the three groups(P0.05).In group B,inflammatory cell infiltration,alveolar block and consolidation of lung and vasodilation in bronchial wall were observed,and the alveolar septa widened,while the inflammatory cell infiltration was reduced and alveolar septa were not widened in group C.Significant pathological changes were not observed in group A.Conclusion:Qinshixunjing patch could obviously relieve inflammatory reaction in lung tissues and bronchus,and reduce the percentage of eosinophils in peripheral blood of asthmatic guinea pigs.