Expression of KLF2 in Lung Tissue of Guinea Pigs with Bronchial Asthma and Its Significance

Objective:To investigate the expression and significance of KLF2 in lung tissue of guinea pigs with Bronchial Asthma.Methods:Thirty healthy male guinea pigs were randomly divided into 4 groups:the control group(group A),asthmatic group(group B),Dexamethasone group(group C),10 guinea pigs in each group.The asthmatic model was established by the ovalbumin challenge method.Before determining the total number of BALF cells and cells classification,the changes in lung histopathology were observed.Expressions of KLF2 mRNA in lung tissue were assayed by in situ hybridization and by RT-PCR;Expressions of KLF2 protein were detected by immunohischemistry and by Western blot.Results:(1)The total number of inflammatory cells and EOS%,NEU% in BALF of asthma group were significantly higher than those of control group(P0.01),and large numbers of inflammatory cell infiltration and obviously bronchiole remodeling response could be seen in this group.However,all these changes were obvious decreased after Dexam-ethasone treatment.(2) Protein and mRNA expressions of KLF2 in asthma group significantly higher than those in control group(P0.01),and those in Dexamethasone group was markedly increased compared to asthma group(all P 0.01).(3) Linear correlation analysis showed that KLF2mRNA and protein was negatively correlated with EOS%,NEU% and the total number of inflammatory cells in BALF(r=0.524,P 0.01).Conclusions:In acute exacerbation of Bronchial Asthma model induced by ovalbumin,the study showed that asth-matic guinea pigs have significantly decreased expressions of KLF2,which up-regulated by Dexamethasone treatment group,suggesting that KLF2 may play an important role in the pathogenesis and prevention of Bronchial Asthma.