Expression of PTEN,p53 and PCNA Proteins in Human Colorectal Carcinoma

【Objective】To investigate the expression of PTEN,p53 and PCNA proteins in colorectal carcinoma and its significance.【Methods】Expressions of PTEN,p53 and PCNA were detected in paraffin-embedded tissues by immunohistochemical technique sp.The specimens included 12 normal colorectal tissues and 78 colorectal carcinoma tissues and surrounding non-tumor colorectal tissues.【Results】Expressions of PTEN in the normal colorectal tissue,the surrounding non-tumor tissue and the colorectal carcinoma tissue were 100%,96%,50%respectively..Expressions of p53 in the surrounding non-tumor tissue and the colorectal cancer tissue were 2%,62%respectively.Expressions of PCNA in the surrounding non-tumor tissue and the colorectal cancer tissue were 3%,76%.A positive correlation was noted between expression of p53 and PCNA(r=0.370,P0.05),and an inverse correlation was noted between expression of PTEN and p53 in colorectal carcinoma(r=-0.610,P0.05).【Conclusion】Down regulation of PTEN expression,p53 mutation and over expression of PCNA may play a role in occurrence of colorectal carcinoma.Associating PTEN with p53 and PCNA can offer a valuable reference to treatment and follow-up for patients after colorectal carcinoma resection.