Plant Regeneration of Strawberry Leaves of "Fengxiang"

[Objective] The purpose was to provide the basis for mass production of strawberry seedling by adopting of leaf tissue culture technology.[Method] With Fengxiang strawberry leaves as explant,its callus was induced with 4 kinds of different culture media,and expanding propagation was performed to on the callus,and the best induction medium was screened.[Result] The medium of MS+6-BA 0.5 mg/L+NAA 0.1 mg/L generated callus the fastest,on which,the induction rate of callus,rooting rate and induction rate of adventitious bud were all the highest,up to 69.4%,10.00% and 11.67% resp.,and the callus volume was bigger,the total number of rooting was the most,the adventitious bud grown flourishingly and leaves were more and big,eventually 543 complete plantlets with roots that could be transplanted to outdoors were acquired,the multiple of proliferation and expanding propagation was up to 50 and the proliferation effect was extremely good.On the medium of MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,the callus induction of was 63.8% and the volume of callus was the largest.[Conclusion] The optimal medium for strawberry leaves on inducing callus,adventitious bud and acquiring plenty of regenerating plantlet eventually was: MS+6-BA 0.5 mg/L+NAA 0.1 mg/L.