2008•Journal of Hebei North UniversityRequires access
Plant Regeneration Induced from Calli of Explants of Kyoho Grapevine
Liu Shou-yi
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Abstract
The Kyoho Grapevine was used to study regeneration.Callus were induced from leaves of grapes,differentiated and regenerated into plantlets through organogenesis.The results showed that with 0.1% HgCl2 to disinfect explants for 7min,the rate of pollution and the rate of browning were moderation,and the survival rate was the highest,which was 30.0%.Best medium for the differentiation was MS+6-BA1.0mg/L+NAA0.01mg/L;Leaf callus induction medium of MS additional 5mg/L 6-BA and 0.5 mg/L NAA of leaf callus induction medium was more appropriate;MS+6-BA0.5mg/L+NAA0.05 mg/L was the most suitable for grape adventitious buds callus tissue regeneration;1/2MS+IBA 0.2 mg/L was the ideal rooting medium,and the root length was moderate,the number of root was large.The initial cultivation regeneration system of Kyoho Grapevines was established.