Different Methods of DNA Extraction from Dry Beet Seeds

The genomic DNA of dry beet seeds of was extracted by SDS, CTAB or alkaline lysis method, and used 1% agarose to determine the purity of DNA and λDNA to judge the content of DNA. The results showed that the content of genomic DNA by adopting SDS or CTAB was higher, and both the DNA extracts were close to the total amount. The content of DNA was lower by using alkaline lysis method. However, the DNA extracted by the all three approaches could meet the requirements of SSR-PCR, without any difference in amplification bands. As it was fast and simple to extract DNA by alkaline lysis method, it would be suitable for extracting massive DNA as well as the PCR reaction without a high demand for the template. However, both SDS and CTAB are fit for an one-off massive extraction of the genomic DNA of beets, and for the PCR reaction with a high demand of the purity DNA.