Study on the in vitro quality and function of pooled buffy coat derived platelets

Objective To investigate the in vitro quality and function of pooled buffy coat derived platelets,and to provide the basis for establishment of the national standard for pooled platelets.Methods The platelets were collected by the buffy coat method.The in vitro quality(platelet yield,residual erythrocytes and leukocytes,volume,and pH value) of the pooled platelets were tested by the KX-21 Sysmex cell counter,the pH monitoring meter,and the ES-1000E thermoanalysis Balance,respectively.Platelet aggregation response to ADP and the activation markers CD62P and PAC-1 in platelet membranes in the two groups(the pooled platelet group,and the apheresis platelet group) were detected by APACT2 aggregometry and flow cytometry,respectively.Results The platelet volume,yield,pH value,residual erythrocyte,and residual leukocytes of the pooled platelets were(270±14)ml,(2.58±0.51)×1011,7.24±0.09,(6.61±0.16)×109,and(0.011±0.008)×109,respectively.The platelet aggregation response to ADP in the two groups were(85.1±9.0) % and(87.9±11.1)%(P0.05).The activation markers CD62P and PAC-1 in platelet membranes of the two groups were(12.3±1.7)% and(11.7±2.1) %(P 0.05),(9.3±1.7)% and(8.9±1.4)%(P 0.05),respectively.Conclusion The in vitro quality of pooled buffy coat derived platelets can meet the national standard(GB18469-2001)for apheresis platelet.The function of pooled platelets is normal.