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2008•Beijing Medical JournalRequires access

Experimental study of aggregation and activation in pooled buffy coat-derived and apheresis platelets

Jinghan Liu

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Abstract

Objective To investigate the difference of aggregation and activation in vitro between pooled buffy coat-derived and apheresis platelets. Methods The platelets were divided into two groups: the pooled buffy coat-derived platelet group and the apheresis platelet group. Platelet aggregation response to ADP in the two groups were detected by APACT2 aggregometry. Activation markers CD62p and PAC-1 on platelets in the two groups were measured by flow cytometry. Results The aggregation response to ADP of platelets in two groups were (84.1±9.0)% vs (87.9±11.1)%(P0.05). The level of activation markers CD62p and PAC-1 on the membranes of platelets in two groups were CD62p (13.4±4.6%, 11.6±2.9%; P0.05) and PAC-1(9.3±3.7%, 8.1±2.9%; P0.05). Conclusions It shows no significant difference of aggregation response to ADP and the level of activation between buffy coat-derived and apheresis platelets.

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What this paper is about

Objective To investigate the difference of aggregation and activation in vitro between pooled buffy coat-derived and apheresis platelets. Methods The platelets were divided into two groups: the pooled buffy coat-derived platelet group and the apheresis platelet group. Platelet aggregation response to ADP in the two groups were detected by APACT2 aggregometry. Activation markers CD62p and PAC-1 on platelets in the two groups were measured by flow cytometry. Results The aggregation response to ADP of platelets in two groups were (84.1±9.0)% vs (87.9±11.1)%(P0.05). The level of activation markers CD62p and PAC-1 on the membranes of platelets in two groups were CD62p (13.4±4.6%, 11.6±2.9%; P0.05) and PAC-1(9.3±3.7%, 8.1±2.9%; P0.05). Conclusions It shows no significant difference of aggregation response to ADP and the level of activation between buffy coat-derived and apheresis platelets.

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Available abstract

Objective To investigate the difference of aggregation and activation in vitro between pooled buffy coat-derived and apheresis platelets. Methods The platelets were divided into two groups: the pooled buffy coat-derived platelet group and the apheresis platelet group. Platelet aggregation response to ADP in the two groups were detected by APACT2 aggregometry. Activation markers CD62p and PAC-1 on platelets in the two groups were measured by flow cytometry. Results The aggregation response to ADP of platelets in two groups were (84.1±9.0)% vs (87.9±11.1)%(P0.05). The level of activation markers CD62p and PAC-1 on the membranes of platelets in two groups were CD62p (13.4±4.6%, 11.6±2.9%; P0.05) and PAC-1(9.3±3.7%, 8.1±2.9%; P0.05). Conclusions It shows no significant difference of aggregation response to ADP and the level of activation between buffy coat-derived and apheresis platelets.

Key concepts: Buffy coat, Platelet, Apheresis, Platelet activation, Flow cytometry, Medicine, Platelet aggregation, Significant difference

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