Study on distribution of NOS positive neurons and FOS expression following cerebral ischemia in rat forebrain

Objective:To observe the changes of NOS distribution and time course of FOS protein expression in different regions of rat forebrain following cerebral ischemia-reperfusion. Methods: NADPH-d histochemical and FOS immunohistochemiacal method were used to explore the reperfusion of 2 h,4 h,8 h,12 h,24 h,48 h after ischemia 30 min in rats model of 4 vessel occlusion. Results: The density of NOS postive neurous and its containing reaction increased in the ischemia groups. FOS protein expression was found with the changes of the time after ischemia reperfusion. Approximately 10%～15% of the NOS/FOS double labelled cells showed in several regions. Conclusion: The results indicate that the distribustion of Nos positive necrons and Fos protein expression are closely related to the reperfusion injury after ischemia.