The expression of FOS protein and NOS in brain followingliver ischemia-reperfusion injury in rats

Objective: To observe the changes of nitric oxide synthase (NOS) and fos protein in injuried brain following liver ischemia-reperfusion injury and evaluate the contribution of NOS and c-fos protein to brain tissue effect. Methods: A model of liver ischemia-reperfusion injury was established by imitation. The contents of NOS and c-fos protein in brain tissue including cortex hippocampus and hypothalamus regions were examined individually by immunohistochemical technique following sham operation (group 1), ischemia 30 min without reperfusion(group 2), reperfusion following schemia 30 min (group 3). 1 hour reperfusion following ischemia 30 min (group 4). 2 hour reperfusion following ischemia 30 min (group 5) and 4 hour reperfusion following ischemia 30 min (group 6). Results: Compared with group1, group2 and group3, the contents of NOS and c-fos protein in brain tissue including cortex hippocampus and hypothalamus regions were little statistic significance, (P0.05). There was obviously statistic significance between group4 and former groups, (P0.05). The increase exists remarkably about the contents of NOS and c-fos protein in hypothalamus compared with former groups in themselves (P0.01).There also was higher level in group5 than the former groups (P0.05), while no changes happen between group5 and group6(P0.05). Conclusion: The data of NOS and c-fos protein in brain tissue including cortex hippocampus and hypothalamus regions vary depending on different time of liver ischemia-reperfusion specially, after reperfusion. NOS and c-fos protein are involved in brain tissue injury following reperfusion of ischemia liver.