Expression of monocyte chemoattractant protein and MCP-1 gene in urothelial carcinoma of renal pelvis and its clinical significance

Objective To investigate the monocyte chemoattractant protein(MCP-1) gene expression of urothelial carcinoma of renal pelvis and adjacent normal tissues and the correlation of the incidence and pathological grading of urothelial carcinoma of renal pelvis.Methods Twenty cases of patients with urothelial carcinoma of renal pelvis(12 cases of male,8 cases of female) were taken the blood,carcinoma tissues and adjacent normal tissues.Thirty cases of non-cancer patients(18 cases of male,12 cases of female) as control group were taken blood samples.Expression of MCP-1 in plasma were detected by ELISA method quantitative determination,and the expression of MCP-1 in urothelial carcinoma of renal pelvis and adjacent normal tissues were investigated by immunohistochemical method.Real-time quantitative PCR was used to detect the expression of MCP-1 RNA.Results MCP-1 in plasma of urothelial carcinoma patients of renal pelvis was(173.4±82.1)pg/ml,higher than that of non-tumor group(91.8±34.6)pg/ml(P0.05).Expression of MCP-1 in high-grade urothelial carcinoma of renal pelvis was(254.1±125.8)pg/ml,while in low-grade urothelial carcinoma of renal pelvis was(151.3±79.5)pg/ml.Immunohistochemistry showed that MCP-1 positive rate in urothelial carcinoma of renal pelvis was 90.0%(18/20),and in adjacent normal tissues was 65.0%(13/20),with significant differences(P0.01).Positive expression rate of MCP-1 in high-grade urothelial carcinoma of renal pelvis was 100.0%(4/4),while in low-grade urothelial carcinoma of renal pelvis was 87.5%(14/16).Total RNA and mRNA levels of MCP-1 in the urothelial carcinoma of renal pelvis were statistically significant different compared with adjacent normal tissues group(P0.05).Conclusion The upregulation of MCP-1 gene expression is likely to play an important role in the incidence and metastasis of the urothelial carcinoma of renal pelvis.