2012•Chinese Journal of Health Laboratory TechnologyRequires access

Determination of lead in blood by graphite furnace atomic absorbance spectrometry with double-component mixed deproteinization

Zheng Jiang

Abstract

Objective:To develop a method for the determination of lead in blood by graphite furnace atomic absorbance spectrometry(GFAAS)with double-component mixed deproteinization.Methods:Adequate amount of blood was collected as samples.After the protein in blood was removed by 5% nitric acid-0.2%Triton X-100 and conducted high speed centrifugal separation(120000 rpm,5 min),lead in the supernatant was determined at 283.3 nm with 2%NH4H2PO4 as matrix modifier by GFAAS.Results:The linear range was 1 μg/L~60 μg/L(r=0.9994)and the detection limit was 0.68 μg/L for lead in blood.The relative standard derivations(RSDs) and the average recoveries of the method were 1.5%~4.3% and 95%~106% respectively.Conclusion:The proposed method was characterized by accurate and reliable result,convenient procedure,short analysis cycle and no digestion process.It was suitable for lead analysis in quantities of blood.

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Key concepts: Absorbance, Chemistry, Detection limit, Chromatography, Graphite furnace atomic absorption, Mass spectrometry, Nitric acid, Matrix (chemical analysis)