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2004•Hebei Journal of Forestry and Orchard ResearchRequires access

TISSUE CULTURE AND RAPID PROPAGATION OF Continus coggyria

Xuemei Chen

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Abstract

Choosing stems with buds as explants,the authors were succeed in the study of the best method for tissue culture and rapid propagation of Continus coggyria.1/2MS+NAA 1.0 mg/L+6-BA 0.8 mg/L+sugar 25 mg/L+agar 6 mg/L was the optimum medium for proliferation of bud clusters. Shoots were rooted on the medium 1/2MS+IBA 1.5 mg/L+sugar 20 mg/L+agar 6 mg/L and the rate of rooting was 85%. Being acclimatized for 20 days, the rooted plantlets were transplanted to substrate in two successive steps,the survival rates of which were 80%,100% repectively.

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What this paper is about

Choosing stems with buds as explants,the authors were succeed in the study of the best method for tissue culture and rapid propagation of Continus coggyria.1/2MS+NAA 1.0 mg/L+6-BA 0.8 mg/L+sugar 25 mg/L+agar 6 mg/L was the optimum medium for proliferation of bud clusters. Shoots were rooted on the medium 1/2MS+IBA 1.5 mg/L+sugar 20 mg/L+agar 6 mg/L and the rate of rooting was 85%. Being acclimatized for 20 days, the rooted plantlets were transplanted to substrate in two successive steps,the survival rates of which were 80%,100% repectively.

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Available abstract

Choosing stems with buds as explants,the authors were succeed in the study of the best method for tissue culture and rapid propagation of Continus coggyria.1/2MS+NAA 1.0 mg/L+6-BA 0.8 mg/L+sugar 25 mg/L+agar 6 mg/L was the optimum medium for proliferation of bud clusters. Shoots were rooted on the medium 1/2MS+IBA 1.5 mg/L+sugar 20 mg/L+agar 6 mg/L and the rate of rooting was 85%. Being acclimatized for 20 days, the rooted plantlets were transplanted to substrate in two successive steps,the survival rates of which were 80%,100% repectively.

Key concepts: Agar, Sugar, Explant culture, Tissue culture, Shoot, Horticulture, Substrate (aquarium), Botany

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