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IDENTIFICATION AND DETERMINATION OF GALLIC ACID IN ERSHIBA WEI BINGLANG PILLS

Le Cai

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Abstract

OBJECTIVE To establish the TLC identification methods for Rhizoma Curcumae Longae,Semen Arecae,Fructus Chebulae,Sabina Przewalskii,Fructus Piperis Longi and Berberis kansuensis Schneid ,and an HPLC for quantitative determination of gallic acid in Ershiba Wei Binglang pills. METHODS TLC and HPLC were adopted.RESULTS The specificitions of TLC were proved. RSD of precision for HPLC was 1.70%,and the mean recovery was 98.4%. Regression equation of standard curve was A =5×10 6 C -1 8058×10 4( r =0.9997).CONCLUSION These methods are simple and credible,and can be used to control the quality of Ershibawei Binglang pills.

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What this paper is about

OBJECTIVE To establish the TLC identification methods for Rhizoma Curcumae Longae,Semen Arecae,Fructus Chebulae,Sabina Przewalskii,Fructus Piperis Longi and Berberis kansuensis Schneid ,and an HPLC for quantitative determination of gallic acid in Ershiba Wei Binglang pills. METHODS TLC and HPLC were adopted.RESULTS The specificitions of TLC were proved. RSD of precision for HPLC was 1.70%,and the mean recovery was 98.4%. Regression equation of standard curve was A =5×10 6 C -1 8058×10 4( r =0.9997).CONCLUSION These methods are simple and credible,and can be used to control the quality of Ershibawei Binglang pills.

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Available abstract

OBJECTIVE To establish the TLC identification methods for Rhizoma Curcumae Longae,Semen Arecae,Fructus Chebulae,Sabina Przewalskii,Fructus Piperis Longi and Berberis kansuensis Schneid ,and an HPLC for quantitative determination of gallic acid in Ershiba Wei Binglang pills. METHODS TLC and HPLC were adopted.RESULTS The specificitions of TLC were proved. RSD of precision for HPLC was 1.70%,and the mean recovery was 98.4%. Regression equation of standard curve was A =5×10 6 C -1 8058×10 4( r =0.9997).CONCLUSION These methods are simple and credible,and can be used to control the quality of Ershibawei Binglang pills.

Key concepts: Gallic acid, Chromatography, High-performance liquid chromatography, Pill, Quality standard, Chemistry, Traditional medicine, Biology

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IDENTIFICATION AND DETERMINATION OF GALLIC ACID IN ERSHIBA WEI BINGLANG PILLS — Research Paper | ScholarLens