Study on the correlation between HPV16/18 and expression of p53 protein、PCNA in cervical carcinoma of Uygur women

Objective: To investigate the relationship between human papillomavirus type 16/18 infection and the expressed products of tumor supressor gene p53 and proliferating cell nuclear antigen(PCNA). Methods: 127 paraffin-embedded specimens of cervix were examined including 64 cases of squamous carcinoma (SCC), 31 cases of cervical intraepithelial neoplasia (CIN) and 32 normal persons. The presence of HPV16/18 was detected by PCR technique, using immunostaining (LSAB) for p53 protein and PCNA overexpression.Results: Frequency of positive HPV16/18 DNA was 50.0% in SCC, 22.5% in CIN and 12.5% in normal controls. p53 expression was 54.7% in SCC, 22.5% in CIN and 6.3% in normal controls The PCNA expressive rates were 81.2%, 62.8%, 6.3% in SCC. CIN and normal cervix respectively. There was a significant difference between SCC and non-neoplastic lesions .Expressions of p53 protein and PCNA were not correlated to the grade, the tumor size, and the lymph node metastasis.Conclusion: ①HPV16/18 infection in Uygur women may have important ehologic significance of cervical carcinogenesis; ②Mutation of p53 tumor suppressor gene and expression of PCNA may have critical role on cervical carcinogenesis: ③There are close association of HPV16/18 infection and over-expression of P53 protein, PCNA with Uygur women cervical SCC could be as a valuable index for early diagnosis, and progonosis of cervical cancer.