2015•Zhongguo linchuang baojian zazhiRequires access
Effects of 17-allylamino-17-demethoxygelda-namycin(17-AAG) on apoptosis of human hepatoma carcinoma cell lines HepG 2 in vivo
Chen Mein
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Abstract
Objective To observe the influence of 17-allylamino-17-demethoxygelda-namycin( 17-AAG) on cell apoptosis of Human hepatoma carcinoma cell Lines Hep G2,and explore the possible mechanism. Methods Cellular morphological alterations were observed after AO / EB staining; the apoptosis and cell cycle of Hep G2 cells were assayed by flow cytometry; the expression of apotosis related proteins bax and bcl-2 in Hep G2 cells treated with 17-AAG were detected by RT-PCR. Results The result of AO / EB double staining showed that the control homogeneously were green fluorescence; the experimental group were red fluorescent,and the red fluorescent was increased with drug concentration gradually; Flow cytometry result showed 17-AAG promote hepatocellular carcinoma Hep G2 cell apoptosis; RT-PCR results showed that 17-AAG can increased bax mRNA expression levels of protein,decreased the BCL-2 mRNA protein expression levels,the effect was related with drug dose. Conclusion 17-AAG can inhibit proliferation and induce apoptosis of Hep G2 cells,and the mechanism may be related to its effect in up-regulating bax protein expression and down-regulating the BCL-2 protein expression.