Analysis of the distribution and drug resistance of pathogenic bacteria in blood cultures

Objective To offer the clinical physician the basis of optimal application of antibiotic,we have investigated the variation of antibiotic resistance and the bacterial distribution in the blood culture from July 2007 to Sept.2009 in the central hospital of Wuhan.Methods Blood was cultured in BACTEC 9050(BD,US).The clinical isolates were identified by API identified tests(Bio-Merieux company of France).Kirby-Bauer method was used in antibiotic sensitivity test,and the results was analyzed by WHONET5.4 software,which was determined by the CLSI standard of the edition in 2007.Results Of 2256 blood samples,the bacteria-positive samples were 269 (11.9%).There were 129 strains of gram-positive cocci accounted for 47.96 %,116 strains of Gram-negative bacilli accounted for 43.12 %.24 strains of fungi accounted for 8.92 %.The antibiotic sensitivity test showed that the sensitivity of the main gram-positive bacilli to vancomycin、 teicoplanin and linezolid were 100%,and Escherichia coli and Klebsiella pneumoniae to imipenem and meropenem were 100%,Pseudomonas aeruginosa and acinetobacter were highly susceptible to cefoperazone/sulbactam.Conclusion The most pathogenic bacteria isolated from blood are conditional pathogens.gram-positive bacilli is a little more than gram-negative bacilli,and the main bacteria was staphylococcus.Drug resistance of isolated pathogenic bacteria in blood cultures is very serious.It is of great importance in guiding the clinical therapy to monitor the change of pathogens and trends of drug resistance.