Bacterial spectra of blood culture and drug resistance

OBJECTIVE To investigate the changes of bacterial spectra and drug resistance of pathogenic bacteria in blood culture,and provide a basis for clinical treatment.METHODS Blood was cultured by Bactec 9120 of BD.The clinical isolates were identified by API and VITEK-Ⅰ of Bio-Merieux of France.Antibiotic susceptive test was done by broth microdilution method and the result which was analyzed by WHONET5.4 was determined by the NCCLS standard of 2005′s edition.RESULTS From Jan 2005 to Dec 2008,1151 strains of pathogenic bacteria were isolated from 4791 clinic blood specimens,of which 576 strains were Gram-positive cocci,accounting for 50.0%,532 strains were Gram-negative bacilli,accounting for 46.2%,25 strains were Candida albicans accounting for 2.2%.The sensitivity of Gram-negative bacteria to imipenem was the highest;the sensitivity of Gram positive bacteria to teicoplanin and vancomycin were the highest.CONCLUSION Drug resistance of isolated pathogenic bacteria in blood cultures is very serious.Monitoring the change of pathogens and trends of drug resistance is very important in guiding the clinical use of antibiotics.