Optimization of ultrasound-assisted extraction of Radix Glehniae polysaccharides using response surface methology

To optimum extraction conditions of ultrasound-assisted technology of polysaccharides from Radix Glehniae by response surface methodology(RSM).Based on single-factor experiment,three extraction parameters as the ratio of water to sample,ultrasonic power and extraction time were selected as casual factors and three-level three-factor Box-Behnken design was applied to determine the effect of central composite experimental design.The optimum ultrasound-assisted extraction conditions were as follows:the ratio of water to sample 19:1,ultrasonic power 460 W,extraction time 23 min.Under this condition,the yield of Radix Glehniae of polysaccharides was 60.15%.Campared with direct-heating extraction rate was improved to about 60%.