An Approach to Normal Range of Blood Routine and Biochemical Indexes of SPF Wistar Rats

ObjectiveTo determine normal ranges of blood and biochemical indexes in laboratory rats for toxicological safety evaluation,and compare the influence of blood extraction by decapitation and by abdominal extraction after anaesthesia on biochemical indexes tests.Method Determine the normal range with 95% confidence level through normal distribution by processing statistics of all blood and biochemical indexes in 2～3 month-old SPF Wistar rats collected over recent 3 years by our laboratory;compare the differences of results from male and female rats and two extraction methods.blood indices.Result(1)There is no difference in WBC,RBC,HGB and PLT counts between males and females,but there is difference in WBC classification between them.(2)Biochemical indexes showed difference between males and females.In results of tests of blood by decapitation ALT,AST,TG in females are lower than in males,but their TP,Alb,TCH are higher than males.Therefore there is statistical significance(P0.05).In results of tests of abdominal blood,ALT,GLU,TG in females are lower than in males and their AST,TP,Alb,BUN,TCH are higher than males and there is statistical significance(P0.05).(3)Methods for blood extraction do have influence on biochemical test results.ALT,AST,TP,Alb,BUN,TCH,TG levels from tests of blood from decapitation are higher than from tests of blood from abdomen after pentobarbital sodium anaesthesia and there is statistical significance(P0.01);GLU,CRE levels from test of blood through decapitation are lower than from the latter method and showed statistical significance(P0.01).Conclusion The normal ranges of blood and biochemical indexes for Wistar rats were therefore established.