Establishment of normal reference range of blood hematological and biochemical indicators of SPF wistar rats

Objective To update and establish the new normal range of blood hematological and biochemical indicators of SPF Wistar rats in toxicological safety evaluation,and to provide an accurate,reliable,scientific basis for the non-clinical toxicological evaluation and scientific research.Methods Blood hematological and biochemical indicators of 8-10 weeks age SPF Wistar rats with no treatment over the past 3 years in the laboratory were collected and analyzed statistically.The normal range with 95% confidence level was calculated through normal distribution model.Difference between male and female rats was compared.Results (1)There was no difference in WBC,RBC,HGB counts between male and female,while there were significant difference in PLT and WBC classification between different sexes(P0.01).(2)Biochemical indicators showed difference between male and female:ALT,TG of the female were lower than that of the male;TP,ALB,A/G of the female were higher than that of the male(P0.05 P0.01).Conclusions The normal range of blood hematological and biochemical indicators of SPF Wistar rats were updated and established.