A Study on the Activities of Amylase and Lipase in Digestive Tract of Rana rugulosa

The distribution of amylase and lipase in the digestive tract of Rana rugulosa and the effects of pH and temperature on the activities of these digestive enzymes were studied by enzymatic methods. The results indicated that the activities of digestive enzymes in different parts of digestive tract were different significantly. The activities of amylase from high to low were the foregut,the midgut,the hindgut,the esophagus and the stomach. The optimum pH values of the amylase in stomach and intestine were 8. 6 and 7. 0 respectively,and their optimum temperature were 35 ℃ and40 ℃ respectively. The activities of lipase from high to low were the midgut,the hindgut,the foregut,the stomach and the esophagus,respectively. The differences between various parts of digestive tract were significant( P 0. 05). The optimum pH values of the lipase in stomach and intestine were equally 9. 0,and their optimum temperature were 50 ℃and 55 ℃ respectively.