Experimental study of H22 hepatoma cell apoptosis induced by interstitial radioactive seeds~(125)I

Objective To investigate the effects and mechanisms of H22 hepatoma cell apoptosis induced by radioactive seeds ~(125)I.Methods The H22 hepatoma cell apoptosis induced by radioactive seeds ~(125)I brachytherapy was measured by Terminal deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling (TUNEL) method,and immunohistochemical stain (Elivion~(TM) plus) was used for detecting the protein expression of Survivin.Sixty mice were randomly divided into 4 groups (n=15 in each group): group A (interstitial radioactive seed ~(125)I),group B (DDP),group C (interstitial radioactive seed ~(125)I plus DDP),group D (control group).Results The tumor volume inhibition rate in groups A,B and C was 43.8%,40.7% and 58.3% respectively.Apoptosis index (AI) in groups A,B and C was (25.15±10.36),(33.42±12.25) and (42.34±13.95) respectively.The AI in group C was significantly higher than in group D (20.45±14.54) (P0.05).The protein expression rate of Survivin in groups A,B and C was 50.0%,55.6% and 36.4% respectively.The expression rate of group D (100%) was significantly higher than in group C (P0.05).Conclusion The seed ~(125)I brachytherapy induces hepatoma cell ap- optosis and inhibits hepatoma cell proliferation.Combined with chemotherapy,~(125)I induces stronger apopto- sis.Survivin may play a role in the regulation of apoptosis.