2012•Acta Agriculturae ZhejiangensisRequires access
AFLP analysis of genetic diversity of Angelica dahurica.
Xiao-Xia Shen, Shen Yu-feng, Jianming Jiang
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Abstract
The 50 germplasmic resources of Angelica dahurica were analyzed by AFLP to determine the genetic diversity.The amplified fragment length polymorphism(AFLP) tags were used to investigate the genetic diversity at the DNA level.Twelve pairs of selective amplification primers were screened out to amplify the bands,which were used as original matrix and analyzed the similarity between different Angelica dahurica germplasm by NTSYS-PC software calculation and constructed the phylogenetic tree.The results indicated that the similarity of the 50 Angelica dahurica germplasm resources all more than 70%,whose overall phylogenetic relationships were relatively close.The germplasm ZYS002039 and ZYS002045 showed larger difference in genetic background from the others.