AFLP Analysis of genetic diversity of Leonurus japonicus germplasm resources

Objective The AFLP analysis of nine accessions of Leonurus japonicus was conducted to explore genetic diversities among germplasm.Methods Genetic diversities at DNA level among the germplasm of L.japonicus were studied by the amplified fragment length polymorphism(AFLP) marker,and 32 pairs of selective amplification primer were identified.The similarities among the germplasm of L.japonicus were calculated and analyzed by the software of NTSYS-PC,and genetic evolution tree was developed,using amplified fragments as primary matrix.Results The quality of genomic DNA of L.japonicus extracted by SDS method was good enough to meet the requirement of AFLP analysis.Ten combinations with multiple morphologies,clear bands,and high discrimination rate were identified from 32 pairs of selective amplification primers.The AFLP fingerprinting was developed,which could differentiate each of nine accessions of L.japonicus.Nine accessions of L.japonicus could be classified into two types based on the evolution tree.Conclusion There are richer genetic diversities among the germplasm resources of L.japonicus,and certain relationships are found between some of morphological characteristics and genotypes.