AFLP analysis on genetic diversity of Sarcandra glabra in Guangxi region

Objective To evaluate the genetic diversity of germphlasm resources for Sarcandra glabra and provide biological reference for germplasm resources protection,genetic relationship analysis,breeding,and introduction and cultivation of S.glabra and other Chinese herbal medicines.Methods Amplified fragment length polymorphism(AFLP) marker was developed to analyze the genetic diversity among 18 samples of S.glabra from various habitats in Guangxi region,and cluster analysis and principal coordinate analysis were revealed by the NTSYS software.Results Eight out of sixty-four AFLP primer combinations selected were used for amplification and a total of 319 unambiguous bands were obtained,among which 251(PPB=78.68%) were polymorphic.The results of cluster analysis and principal coordinate analysis showed that there was a certain genetic basis of diversity in S.glabra.The genetic similarity coefficients(0.630 3—0.836 6) were bigger while the genetic distance was closer.Conclusion The genetic diversity of germplasm resources for S.glabra in Guangxi region is not abundant and protection strategies should be carried out on S.glabra.