2007•Zhongguo yaofangRequires access

Determination of Protocatechuic Acid in Wuchuishugan Capsules by RP-HPLC

Dong Ping

Abstract

OBJECTIVE:To establish a RP-HPLC method for the determination of Protocatechuic acid in Wuchuishugan capsules.METHODS:The determination was performed on DiamonsilTM C_(18)column(250mm×4.6mm,5μm)with column tem- perature at 30℃.The mobile phase was methaol-0.02%H_3PO_4(15:85)with a flow rate of 1.0mL·min~1.The detection wavelength was 260nm.RESULTS:The linear range of protocatechuic acid was 5.6～112ng(r=0.9999),and its average re- covery was 98.44%,RSD=2.98%.CONCLUSION:This method is simple,accurate,feasible and reproducible.It can be used for the quality control of Wuchuishugan capsules.

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Key concepts: Protocatechuic acid, Chromatography, Chemistry, High-performance liquid chromatography, Linear range, Linear relationship, Content determination, Mathematics