Study on Regenereration System of Tomato Accessions ‘0946’ and ‘0949’
Zhang Hong-yan
Abstract
Zhang Hong-yan
Abstract
Tomato accessions'0946','0949' were used as test materials for establishing tomato veyeneration system,MS media was used as basic media,calli induction,shoot differentiation and rooting condition of different explants and different hormone combinations were investigated.The results showed that MS+2.0 mg/L 6-BA+0.1~0.2 mg/L IAA was the optimal medium for calli induction and adventitious bud differentiation,MS+0.2 mg/L IAA was the optimal medium for rooting.This study not only establish the regeneration system for tomato accessions ' 0946' and' 0949',but also provide technological basis for tomato genetic transformation.
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Tomato accessions'0946','0949' were used as test materials for establishing tomato veyeneration system,MS media was used as basic media,calli induction,shoot differentiation and rooting condition of different explants and different hormone combinations were investigated.The results showed that MS+2.0 mg/L 6-BA+0.1~0.2 mg/L IAA was the optimal medium for calli induction and adventitious bud differentiation,MS+0.2 mg/L IAA was the optimal medium for rooting.This study not only establish the regeneration system for tomato accessions ' 0946' and' 0949',but also provide technological basis for tomato genetic transformation.
Key concepts: Explant culture, Transformation (genetics), Biology, Shoot, Botany, Regeneration (biology), Tissue culture, Horticulture