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2011•Northern HorticultureRequires access

Comparison in Plant Regeneration of Different Varieties of Tomato

Meiping Zhang

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Abstract

Using three kinds of tomatoes as material,7 old days in vitro seedling cotyledon and hypocotyls were used as explant to filter genotype,differential medium and root medium in order to establish tomato regeneration system.The next step was using ginseng large DNA fragment transform tomato.The results showed that 'Fenguan 888'could provide lots of and stable explant,the suitable differentiation medium was MS+6-BA 1.0～2.0 mg/L+IAA 0.2 mg/L,and the suitable rooting medium was MS+IAA 0.5 mg/L.

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What this paper is about

Using three kinds of tomatoes as material,7 old days in vitro seedling cotyledon and hypocotyls were used as explant to filter genotype,differential medium and root medium in order to establish tomato regeneration system.The next step was using ginseng large DNA fragment transform tomato.The results showed that 'Fenguan 888'could provide lots of and stable explant,the suitable differentiation medium was MS+6-BA 1.0～2.0 mg/L+IAA 0.2 mg/L,and the suitable rooting medium was MS+IAA 0.5 mg/L.

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Available abstract

Using three kinds of tomatoes as material,7 old days in vitro seedling cotyledon and hypocotyls were used as explant to filter genotype,differential medium and root medium in order to establish tomato regeneration system.The next step was using ginseng large DNA fragment transform tomato.The results showed that 'Fenguan 888'could provide lots of and stable explant,the suitable differentiation medium was MS+6-BA 1.0～2.0 mg/L+IAA 0.2 mg/L,and the suitable rooting medium was MS+IAA 0.5 mg/L.

Key concepts: Explant culture, Hypocotyl, Cotyledon, Seedling, Regeneration (biology), Horticulture, Biology, Botany

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