The effects of levodopa on antioxidation function of parkinson disease rat

ObjectiveTo observe the effects of levodopa on nigral antioxidation system and mitochondrial respiration-chain function, and study its mechanism.Methods The model rats for PD were established through 6-OHDA. The success 16 model rats were randomly divided into 2 groups:Model group(MG), levodopa group (LG), In addition, the normal group was set. Every group was given relevant treatment for 45 days. All rats behavior were tested before and after giving medicine . The activity of GSH-Px, contents of MDA, ROS and Mitochondrionase complex I of nigra were assayed after giving medicine .Results (1)LG decreased rotation behavior (P0.01).(2)Levodopa reduced GSH-Px activity, improved ROS and MDA contents (all P0.05). (3)Complex I content of LG decreased compared with MG (P0.05). ConclusionL-dopa could obviously decrease rotation behavior, but aggravate oxidative stress of nigra, restrain the activity of mitochondrial respiration-chain,Which indicate that long-time taking L-dopa maybe aggravate the progressive lack of nigral dopaminergic neurons.