2011•Zhongguo yaolixue tongbaoRequires access
Establishment and optimization of acute blood stasis rat model
Jin‐Ao Duan
Open publisher page 13 citations
Abstract
Aim To establish and optimize an acute blood stasis rat model which is suitable for evaluating the effects of traditionalChinese medicine for activating blood circulation and dissipating blood stasis.Methods Orthogonality experimental design of adrenline dosage,ice bath time,blood collection time,and rat gender at three different levels was used to establish acute blood stasis rat models.Indexes of haemorheology were used to optimize the model.Furthermore,the effect of Fo-Shou-San,composite danshen dripping pill(CDDP),and aspirin on activating blood circulation and dissipating blood stasis were evaluated using the optimized model.Results The optimum method for making acute blood stasis rat model was as follows: 0.8 mg·kg-1 adrenline was injected subcutaneously into female rat,time in ice bath was 4 min,and blood was collected at 12 h after the last injection of adrenline.Fo-Shou-San,CDDP,and aspirin showed good improving effect of haemorheology on the optimum model.Conclusion Adrenline dosage,ice bath time,blood collection time and rat gender are the key factors for replicating the acute blood stasis rat model.