Phenolic compounds from Sargentodoxa cuneata (Oliv.) Rehd.et Wils.and their antitumor activities

Aim To clarify the anticancer constituents from Sargentodoxa cuneata (Oliv.)Rehd.et Wils.,a Chinese folk medicine used in the treatment of cancers.Methods The separation procedure was guided by a bioassay using tsFT210 cells.Vacuum liquid column chromatography over silica gel,column chromatography over Sephadex LH 20,and HPLC were employed for the isolation and purification of the bioactive compounds.The structures of the compounds were identified by the spectroscopic method.And the anticancer activities were assayed by the SRB method and flow cytometry.Results Seven phenolic compounds were isolated and identified as 3,5 O dimethyl gallic acid(1),protocatechuic acid(2),chlorogenic acid(3),7 (4 hydroxy 3 methoxyphenyl N [7′ (4′ hydroxyphenyl)ethyl] E 8 propenamide(4), p hydroxyphenethyl alcohol(5),(-) epicatechin(6)and procyanidin B 2[epicatechin (4 β →8) epicatechin](7).Conclusion Compounds 1,3,4,5,6 and 7 are isolated for the first time from this genus.Compound 7 inhibited the cell cycle progression of tsFT210 and K562 cells at the G 2/M phase.Compounds 3 and 4 inhibited the proliferation of K562 cells with the IC 50 value of 97 2 μg/mL for compound 3 and with the inhibition rates of 46 6% at 100 μg/mL for compound 4.