Analysis of correlation between antibiotic resistance and integron gene of Klebsiella pneumoniae

OBJECTIVE To study integron prevalent status of Klebsiella pneumoniae and the relevance to drug-resistance. METHODS The clinical isolates of K.pneumoniae were collected.Drug resistance was determined by K-B disk diffusion method.K.pneumoniae integron gene was amplified by polymerase chain reaction(PCR);Products were detected by agar gel electrophoresis.The relationship between the existence of integron genes and their drug-resistance was analyzed finally. RESULTS Among 115 strains of K.pneumoniae,integron gene were found in 42.6%(49/115).The gene-positive strains showed higher drug resistance,especially in mikacin,ceftazidime,cefotaxime,cefuroxime,piperacillin,piperacillin/tazobactam,trimethoprim/sulfamethoxazole and levofloxacin antibiotic resistance. CONCLUSION There is tightly relationship between the existence of integron genes and drug resistance;integron plays an important role in drug resistance.