2005•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of SOD and Amino Acids in Ge-enriched Mycelia of Ganoderma lucidum

Li-xiong Feng

Abstract

Objective:To determine the contents of SOD and amino acids in Ge-enriched mycelia of Ganoderma lucidum and explore the optimum dosage of GeO_2 in culture medium.Methods:The contents of amino acids were analyzed by high performance liquid chromatography and SOD was determined by nitroblue tetrazolium photoreduction method.Results:Adding GeO_2 400 mg·L~(-1) to the culture medium the contents of amino acids and SOD in Ge-enriched mycelia of Ganoderma lucidum increase by(54.34)% and 16.73% respectively over normal mycelia of Ganoderma lucidum.Conclusion:It provides a firm foundation for application of Ge-enriched mycelia of Ganoderma lucidum.

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Key concepts: Ganoderma lucidum, Mycelium, Ganoderma, Chemistry, Amino acid, Food science, Mushroom, Biochemistry