2011•Zhongguo yiyuan ganranxue zazhiRequires access
Investigation of acquired resistance genes to β-lactams,aminoglycosides and mobile genetic elements in multidrug-resistant Escherichia coli
Jin Yue
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Abstract
OBJECTIVE To investigate the distribution of acquired resistance genes to β-lactams,aminoglycosides and mobile genetic elements in multidrug-resistant Escherichia coli(E.coli). METHODS From Jan 2007 to Dec 2007,20 strains of E.coli were collected from The Second People′s Hospital of Huaian.Then,42 kinds of acquired resistance genes to β-lactams,aminoglycosides and 10 kinds of genetic markers of mobile genetic elements were analyzed by PCR.In addition,sample cluster analysis was performed. RESULTS In 20 strains of multidrug-resistant E.coli,acquired resistance genes to β-lactams: TEM-1,CTX-M-55,acquired resistance genes to aminoglycosides: aac(3)-Ⅱ,aac(6′)-Ⅰb,ant(3″)-Ⅰ,aadA5,aph(3′)-Ⅰ,genetic markers of mobile genetic elements: intⅠ1,tnp513,IS26,ISEcp1,traA,trbC were detected.However,the rest 39 kinds of genes couldn't be detected. CONCLUSION Two kinds of acquired resistance genes to β-lactams and 5 kinds of acquired resistance genes to aminoglycosides might play a role in resistance to β-lactams and aminoglycosides in this 20 strains of multidrug-resistant E.coli.And horizontal transfer of mobile genetic elements brought rapid spread of resistance of bacterial pathogens,not only among the same kinds of pathogens,but among the different ones.In addition,sample cluster analysis shows that no clone transmission was found in this group of E.coli.