Expression and significance of found in inflammatory zone 1 in pulmonary fibrosis of experimental rats

Objective To study the expression and significance of found in inflammatory zone 1( FIZZ1) in pulmonary fibrosis of experimental rats. Methods Ten Sprague-Dawley rats were randomly divided into control group( n = 5) and model group( n = 5). Pulmonary fibrosis model was induced by using the method of intratracheal instillation of bleomycin( 5 mg·kg1). Control group was treated by intratracheal instillation of saline( 1. 25 mL·kg1) to obtain the negative control. On the twenty-eighth day,all rats were sacrificed and the lung tissues were harvested. The lung tissue was performed with hematoxylineosin staining. The severity of pulmonary fibrosis in rats was assessed by Ashcroft score. Immunohistochemical staining was used to detect the expression of FIZZ1 and transforming growth factor-β1( TGF-β1). Results Compared with the rats of control group,the severity of pulmonary fibrosis was increased in rats of model group,and there was significant difference in Ashcroft score( P 0. 01). The expression levels of FIZZ1 and TGF-β1 in model group were higher than those in control group( P 0. 01). Conclusion FIZZ1 may play an important role in the development of pulmonary fibrosis,impossibly relates with TGF-β1 overexpression.