2007•Zhongguo mianyixue zazhiRequires access
The effect of interferon-γ on bleomycin-induced pulmonary fibrosis and expression of interferon-γ and transforming growth factor-β in rats
Zhang De
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Abstract
Objective:To investigate the effects of interferon-γ on bleomycin-induced pulmonary fibrosis in rats and to study the mechanism of its effects.Methods:60 SD rats were randomly divided into 3 groups of 20:normal control group(Group N), model control group(Group M) and interferon-γ(IFN-γ)treated group(Group R). The pulmonary fibrosis model was established by a single intratracheal injection of 5 mg/kg of bleomycin. The rats were sacrificed at days 3,7,14,28 after modeling in batch. Lung tissues were restored to analyze the pathological changes, the content of hydroxyproline,mRNA expression of IFN-γ and transforming growth factor-β(TGF-β).Results:The scores of alveolitis in Group M and R were higher than that in Group N at each poin-in-time after modeling.The scores of alveolitis in Group R were higher than that in Group M. The scores of pulmonary fibrosis and the content of hydroxyproline in lung tissues in Group M and R were higher than that in Group N at day 14 and 28. The expression of IFN-γ mRNA in lung tissues at day 3,7,14,28 and TGF-β mRNA at day 3 in Group R were higher than that in Group M(P0.05).Conclusion:Interferon-γ could not decrease alveolitis and pulmonary fibrosis in early stage of lung fibrosis in rats with bleomycin-induced pulmonary fibrosis,the mechanism might be related to its enhancement on expression of IFN-γ and TGF-β.