Establishment of mice acute liver injury model induced by carbon tetrachloride and it' s application in health food screening

Objective To establish the mice acute liver injury model induced by carbon tetrachloride and to apply in health food screening. Methods Carbon tetrachloride ( CCl4) 1.0 ml/100 g. b. w was given to Kunming mice by abdominal injection at the dose of 0.063% , 0. 125% , 0. 25% , 0.5% and 1.0% for one time. Mice ALT and AST in serum were analyzed 24 hours after administration. Mice liver was observed with routine pathology technique, to determine the liver protection function to mice. Results The serum level of ALT and AST increase significantly in 0.125%, 0.25% , 0.5% and 1.0% CCl4 groups compared with the control group (P 0.05 ) . The pathological changes in 5 dosage groups were balloon degeneration and necrosis mainly, in which, 0.125% , 0.25% , 0.5% and 1.0% CCl4) groups were remarkably different with the control group ( P 0.05) . The sample tested had certain liver protection function attacked with CCl4 at dose of 0.125% to mice. Conclusion 0.125% CCl4 mice acute liver injury model was established and could be applied to health food screening.