Comparison of canine liver failure models induced by carbon tetrachloride and acetaminophen

Objective To investigate the similarities and differences between carbon tetrachloride(CCl4) and acetaminophen(APAP) induced canine liver failure models,and to supply experimental data for studies of drug-related hepatitis.Methods 24 dogs were randomly divided into CCl4 group and APAP group.Dogs in CCl4 group were injected intraperitoneally with CCl4 mixed with the same amount(50%,W /W) of peanut oil in the dosage of 0.9 mL per kilogram of body weight.Dogs in APAP group were given three subcutaneous injections of APAP in dimethyl sulfoxide(600 mg /mL),with the first dose of 750 mg /kg body weight,the second and third dose of 200 mg /kg body weight separately at 9 h and 24 h after the initial injection.The general condition,survival time,the biochemical and hematological indexes(alanine aminotransferase,aspartate aminotransferase /alanine aminotransferase AST /ALT,serum total bilirubin,prothrombin time,blood ammonia,blood urea nitrogen)were measured.The hepatic pathological changes were observed on 15th d or when the dogs died.Results There was no significant difference between the survival rates of two groups(P 0.05).The biochemical and hematological indexes grew to peak in three days.CCl4 could significantly affect liver function(P 0.05),while APAP had more serious renal toxicity(P 0.05).Dogs in CCl4 group showed more serious hepatitis than APAP group.Also the hepatic pathological changes were significant in group CCl4.After CCl4 injection,dogs showed significant changes in neural symptom immediately and in mental state and diet in the following days.Conclusion CCl4 could significantly affect the general condition and liver function of dog more seriously than APAP.However APAP had more serious nephrotoxicity than CCl4.