Establishment of carbon tetrachloride-induced acute liver injury model in mice

Established the model of carbon tetrachloride-induced acute liver injury in mice by investigating the optimal exposure pathway,dose and time.Mice were prepared with 1% CCl4 by intraperitoneal injection and gavage respectively.Serum transaminase levels were detected and liver lesions were examined after 24 h.The results showed that individual difference of serum transaminase level in gavage group was smaller than intraperitoneal injection group,liver lesions were more uniform.Optimal dose and time was investigated by gavage because it has the same poisoning way with reality.Mice were given carbon tetrachloride in oil solution by gavage,with concentration of 0.125%,0.25%,0.35% and 0.5%.Detected serum transaminase levels after 24 h to measure optimal exposure dose.Serum transaminase levels was detected at 2,6,12,16,20,24,28,32,48 h after mice were expoed to optimal dose of CCl4.The results showed that mice received 0.35% CCl4 serum transaminase had significant difference compared with control group(P0.01).Serum transaminase level had significant difference after 20 h(P 0.05).The maximum turned out at 24 h,this level had significant difference compared with control group(P0.01).The ideal model of acute liver injury in mice could be set up by gavage 0.35 % carbon tetrachloride,0.1 mL·10 g-1,for 24 h.