Influence of Kang Naoye on stem cell factor mRNA expression after focal cerebral ischemia-reperfusion injury

Objective Toobservetheinfluence of Kang Naoye on the expression of stem cell factor (SCF) mRNA after focal cerebral ischemia-reperfusion injury of different times and different positions in rats.Methods A middle cerebral artery occlusion (MCAO) model was established with the intraluminal suture method.Adult SD rats were randomly divided into model group,medicine intervention group and sham-operation group.The expression of SCF mRNA after 1.5-hour ischemia and 1-day～14-day reperfusion was measured by in site hybridization in different positions of brain.Results After focal cerebral ischemia-reperfusion,the level of SCF mRNA expression in medicine intervention group and model group was obviously higher than that in sham-operation group in the cortical area and corpora striatum area,and peaked at 7 days and dropped at 14 days.Conclusion The expression of SCF mRNA had the same rule in different positions of rats' brain after focal cerebral ischemia-reperfusion.This medicine may promote the effect of proliferation of endogenous neural stem cells.Two groups of SCF mRNA expression was of no significant difference.