The establishment of renal acute rejection model in rats

Objective To establish the model of rat renal acute rejection and investigate the mechanism of transplantation immunology.Methods Normal Wistar male rat kidney were used as control.Wistar rat which received Wistar rat renal grafts were used as syngenegic group(sTX).Wistar rat which received SD rat renal grafts were used as allogenegic group(aTX).Wistar rat which received SD rat renal graft with CsA treatment were used as immunosuppression group(aTX +CsA).Grafts were harvested at day 3,5,7 and performed HE,Masson,PAS,PASM staining.AR semiquantitative scores were analyzed by using microscope according to the standard of Banff 97.Results Compared with the normal control,AR semiquantitative scores increased significantly in aTX group at the day 3,5,7 after transplantation(P0.01);the score increase a little bit in sTX group,but there is no significant difference compared with control group(P0.05).AR semiquantitative score increased significantly in aTX group at the day 3,5,7 after transplantation compared with sTX group(P0.01).The score increased significantly at the day 5,7 after transplantation compared the scores at day 3 in aTX group(P0.05).No significant different can be found between sTX group and aTX+CsA group at the day day 3,5,7 after transplantation.Conclusion Only slightly rejection can be found in sTX group,this group can be used as the control of rat renal AR;AR can be found in SD renal grafts,so SD to Wistar renal transplantation can be used for rat renal graft AR model;CsA treatment can reduce and suppress renal graft AR.