Chemical constituents from the flowers of Castanea mollissima Blume

Objective To isolate the chemical constituents and elucidate their structures from the flowers of Castanea mollissima Blume.Methods The compounds were isolated by column chromatography on silica gel,Sephadex LH-20 and so on,together with recrystallization methods.NMR spectral analysis and co-TLC were employed for the structural identification.Results Eleven compounds were obtained and elucidated as:2α,3β,23-trihydroxy-olean-12-en-28-acid(1),4-quinolinone-carboxylic-2-acid n-butyl ester(2),quercetin-3-O-β-D-galactopyranoside(3),kaempferol(4),quercetin(5),kaempferol-3-O-[6″-O-(E)-p-coumaroyl]-β-D-glucopyranoside(6),kaempferol-3-O-[2″,6″-di-O-(E)-p-coumaroyl]-β-D-glucopyranoside(7),gallic acid(8),protocatechuic acid(9),5-hydroxymethylfurfuraldehyde(10),quercetin-3-O-β-D-glucuronide 6″-methyl ester(11),respectively.Conclusions Compounds 1,2 and 11 are isolated from Castanea genus for the first time,and compound 3 is obtained from this plant for the first time.