Isolation and identification of chemical constituents from Castanea mollissima Blume

Objective To isolate and identify the compounds from the nbutanol fraction of 90% EtOH extract of seeds of Castanea mollissima Blume.Methods Compounds were isolated by the silica gel and Sephadex LH-20 columns,and their structures were identified by the physicochemical characters and spectral analysis.Results Six compounds were obtained and identified as hexadecanoic acid 2,3-dihydroxypropyl ester(1),maltose (2),D-glucose(3),D-fructose(4),5-hydroxymethylfurfural(5) and kaempferol(6).Conclusions Compounds 1 and 5 are obtained from Castanea genus for the first time and compounds 2,3,4,6 are obtained from the plant for the first time.