Depression Effect of Kangxianling on Transforming Growth Factor-β and CollagenI of Bone Marrow-derived Fibroblast

Objective To observe the effect of Kangxianling on transforming growth factor-β(TGF-β) and collagenⅠ of bone marrow-derived fibroblast.Methods 3.2 g/mL Kangxianling and 0.33 mg/mL fosinopril was administered respectively to rats by gavage(distilled water to normal group) to prepare Kangxianling serum,fosinopril serum and normal serum.The serum was diluted with DMEM medium and divided into normal serum group,fosinopril group,Kangxianling group,TGF-β1 group,TGF-β1+fosinopril group and TGF-β1+Kangxianling group.Bone marrow-derived fibroblast was dissociated and harvested by trypsin,which was isolated from the normal rat kidney tissue.The expression of TGF-β and collagenⅠ of bone marrow-derived fibroblast were examined by enzyme-labeled immunosorbent assay and real time fluorescent quantitation polymerase chain reaction.Results TGF-β and collagenⅠ of bone marrow-derived fibroblast with TGF-β1 stimulating factor increased markedly than those in normal serum group,and were inhibited by Kangxianling and fosinopril.Conclusion Kangxianling could depress the expression of TGF-β,reduce collagenⅠ production of extracellular matrix,thereby delaying the process of renal fibrosis.