Effect of Kangxianling on Phenotypic Transformation of Bone Marrow-Derived Fibroblast

Objective To study the effect and mechanism of Kangxianling on renal interstitial fibrosis (RIF).Methods Kangxianling serum, fosinopril serum and normal serum was prepared respectively with intragastric administration of 3.2g/ml Kangxianling, 3.3mg/ml fosinopril and distilled water to rats. The serum was diluted with a DMEM medium and divided into the normal group, fosinopril group, Kangxianling group, transforming growth factor-β1 (TGF-β1) group, TGF-β1+fosinopril group and TGF-β1+Kangxianling group. The bone marrow-derived fetal bovine serum (FBS) was studied. The expressions of α-smooth muscle actin (α-SMA) and Collagen I were examined by enzyme-linked immunosorbent assay (ELISA) and real time polymerase chain reaction (RT-PCR).Results Comparing with the normal group, the expressions of α-SMA, Collagen I, α-SMA mRNA and Collagen I mRNA were significantly improved in the TGF-β1 group (P0.05 or P0.01). Comparing with the TGF-β1 group, the expressions of α-SMA, Collagen I, α-SMA mRNA and Collagen I mRNA were significantly decreased in the TGF-β1+fosinopril group and TGF-β1+Kangxianling group (P0.05 or P0.01).Conclusion fKangxianling can antagonize RIF by inhibiting phenotypic transformation of bone marrow-derived FBS.