The Effect of Zhixiaotongmaining on the Expression of VEGF mRNA of Human Renal Tubular Epithelial Cell HK-2 Induced by TGF-β_1

Objective:Through studying the effect of Zhixiaotongmaining (ZXTMN) on the expression of VEGF mRNA of human renal tubular epithelial cells HK-2 induced by transforming growth factor-β1(TGF-β1),to explore the mechanism of ZXTMN on the prevention and treatment of renal fibrosis.Methods:The HK-2 cells were cultured by DMEM/F12(1∶ 1) with 10 % fetal bovine serum and divided into control group,TGF-β1 group (TGF-β1 10 ng/ml),animal blank serum control group(TGF-β1 10 ng/ml+10% animal serum),ZXTMN drug-containing serum therapy groups (TGF-β1 10 ng/ml+10% low dose ZXTMN、TGF-β1 10 ng/ml+10 % middle dose ZXTMN、TGF-β1 10 ng/ml+10% high dose ZXTMN).After 24 h,the expression of VEGF mRNA was tested by fluorescence quantitatiye PCR assay.Results:After the HK-2 cell was induced by TGF-β1,the expression of VEGF mRNA was much increased compared with the control(P0.05).But the expression of VEGF mRNA was decreased compared with TGF-β1 group (P0.05).Conclusion:ZXTMN could prevent the development of renal fibrosis to a certain extent.